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Fig. 3 | Phytopathology Research

Fig. 3

From: Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype

Fig. 3

Analyses of the Rx gene in PVX-resistant potato cultivars. a PCR detection of the Rx gene using primer pair RxSP-S3 and RxSP-A2. M, DL2000 DNA marker (Vazyme). b Multiple sequence alignment of the amino acid sequences of Rx1, Rx2 and their paralogs from Longshu-3, Eugene, Atlantic and Waiyin-2. The conserved identical amino acids are highlighted in black. Sequences were aligned with Clustal Omega and displayed by ESPript 3.0. c Phylogenetic analysis of Rx1 and its paralogs. The phylogenetic tree was constructed using the Maximum Likelihood method with 1000 bootstrap replicates and rooted to Ry (Genbank accession no. QEL52752)

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