Fig. 1From: Three highly sensitive monoclonal antibody-based serological assays for the detection of tomato mottle mosaic virusRT-PCR identification and electron microscopic observation of purified ToMMV virions. a The ToMMV infection in tomato plants used for virion purification was confirmed by RT-PCR. b Electron micrograph of purified ToMMV virions. ToMMV virions were purified from ToMMV-infected tomato leaf tissues. The negative staining of virus particles was performed using 1% phosphotungstic acid solution, the samples were subsequently examined and imaged under an electron microscope. Bar = 5 µmBack to article page