Fig. 3

BcAtg2 is essential for autophagy in B. cinerea. a Subcellular localization of GFP-BcAtg8 in the wild-type strain B05.10 and the BcATG2 deletion mutant ΔBcAtg2 with or without nitrogen-starvation treatment. Vacuoles were stained with CMAC (7-amino-4-chloromethylcoumarin). b Analysis of proteolysis of GFP-BcAtg8 in the wild-type strain B05.10 and ΔBcAtg2 with or without nitrogen-starvation treatment using anti-GFP Western blot. GAPDH was used as an internal reference. c The percentage of free to total GFP. Error bars denote standard deviations from three independent experiments, and asterisks indicate statistically significant differences at P = 0.05, NS indicates no significant difference