Fig. 1

The cyp707a mutants display reduced PTI response. a and b flg22- and elf18-induced ROS burst is reduced in cyp707a mutants. Leaf discs from three-week-old Arabidopsis plants were treated with 100 nM flg22 or 100 nM elf18, and the ROS burst was recorded. H₂O served as a control. Error bars represent means ± SD (n = 3 biological replicates). RLU, Relative light units. c and d cyp707a mutants display decreased callose deposition in response to flg22 treatment. Four-week-old Col-0 and cyp707a mutants were syringe infiltrated with Mock (10 mM MgCl₂) or 100 nM flg22. The calloses were stained with aniline blue (c) and counted under microscope (d) at 12 hpi (hours post-inoculation). Scale bar = 100 μm. Error bars represent means ± SD (Two-way ANOVA, P < 0.001, n = 4)