Skip to main content
Fig. 3 | Phytopathology Research

Fig. 3

From: The Pseudomonas syringae effector AvrPtoB targets abscisic acid signaling pathway to promote its virulence in Arabidopsis

Fig. 3

AvrPtoB interacts with CYP707As. a AvrPtoB interacts with CYP707As in N. benthamiana by split luciferase assays. N. benthamiana leaves were co-infiltrated with 35S:cLUC-AvrPtoB and 35S:CYP707A1-nLUC or 35S:CYP707A2-nLUC. Luciferase complementation imaging assays were performed 48 h later. This experiment was repeated three times with similar results. RLU, Relative light units. The combination of 35S:C2-nLUC and 35S:cLUC-S1 was used as a positive control. b and c AvrPtoB interacts with CYP707As in vivo by co-immunoprecipitation assays. N. benthamiana leaves were co-infiltrated with 35S:CYP707A1-HA or 35S:CYP707A2-HA and 35S:AvrPtoB-FLAG or 35S:GFP-FLAG. Plant leaves were immunoprecipitated with anti-FLAG beads, and the proteins were immunoblotted by anti-FLAG antibody. Co-IP proteins were immunoblotted by anti-HA antibody

Back to article page

Phytopathology Research

ISSN: 2524-4167