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Fig. 2 | Phytopathology Research

Fig. 2

From: Systemic necrosis induced by overexpression of wheat yellow mosaic virus 14K suppresses the replication of other viruses in Nicotiana benthamiana

Fig. 2

WYMV 14K protein triggers systemic necrosis and inhibits TRV systemic infection in N. benthamiana. a Schematic diagram of the positions of 1-9 deletion mutations and 14K sequences corresponding to the WYMV genome. b Symptoms of overexpression of WYMV 1-9 mutants in N. benthamiana at 5 dpi. OE-GUS was used as a negative control. The necrosis sites in leaves are indicated by white arrows. c Symptoms of overexpression of GUS or 14K at 5 dpi in N. benthamiana. The necrosis sites in leaves and stem are indicated by white arrows. d Relative transcript levels of TRV CP in systemic leaves of N. benthamiana plants agro-inoculated with OE-GUS or OE-14K. **, P < 0.01. e Detection of the expression of OE-GUS and OE-14K in infiltrated leaves through immunoblotting. The positions of expected protein bands are indicated with black arrows and Ponceau S staining showed equal loading. f Co-localization of 14K-GFP with the ER marker (mCherry-HDEL) at 48 hpi. Bar, 25 μm

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Phytopathology Research

ISSN: 2524-4167