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Fig. 1 | Phytopathology Research

Fig. 1

From: Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus

Fig. 1

Schematic diagram of the method for rapid and visual nucleic acid detection using RT-RAA-CRISPR/Cas12a assay. In this detection system, target gene fragments were amplified with RAA procedure; ssDNA probes (5′-FAM/3′-BHQ1 labeled) were added, and subsequently cleaved by Cas12a to generate green fluorescence; the detection results were visible to the naked eye under blue light

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Phytopathology Research

ISSN: 2524-4167