Fig. 3

BcSec4 is required for acidogenicity of B. cinerea. a Medium acidification by B05.10, the ΔBcSEC4 mutants, and the complemented strain. The tested strains were grown on solid complete medium supplemented with a pH indicator. The color change from purple to yellow indicates acidification (pH < 6.0). b PH value determined after incubated in YEPD liquid medium for 48 h. Data are represented as means ± standard deviations (SDs) from three independent experiments. Different letters indicate statistically significant differences (P < 0.01)