Fig. 1

The expression and phylogenetic analysis of FocGCS. a Phylogenetic tree of putative GCS proteins identified in different organisms. Values on the branches of clusters represent the results of bootstrap analysis (10,000 bootstrap replicates). Verticillium dahliae (XP_009653962.1); Colletotrichum gloeosporioides (EQB52767.1); Beauveria bassiana (KAF1734959.1); Pyricularia oryzae (XP_003710106.1); Fusarium graminearum (XP_011324566.1); Fusarium oxysporum f. sp. cubense tropical race 4 (black triangle, XP_031069237.1); Candida albicans (KGU07664.1); Puccinia striiformis (KNF02135.1); Cryptococcus neoformans (AAX55972.1); Ustilago maydis (XP_011390830.1); Rhizoctonia solani (CUA72326.1); Homo sapiens (NP_003349.1); Arabidopsis thaliana (At2g19880). b Transcript levels of FocGCS at different fungal developmental stages. Asterisks indicate significant difference from the mycelia group (Student’s t-test, *P < 0.05, ***P < 0.001). c Transcript levels of FocGCS in infected banana roots. The FocEF1α gene was used as an internal control to normalize the data for comparing the relative transcript abundance. Asterisks indicate significant differences from the control group (Student’s t-test, *P < 0.05, **P < 0.01, and ***P < 0.001). The mean values ± SD were calculated from three independent experiments