Fig. 2

Southern blot identification and growth phenotype of the ∆FocGCS mutant. a Southern blot analysis of genomic DNA extracted from the WT and three putative ∆FocGCS strains and digested with XbaI. b Colony morphology of the WT, ∆FocGCS, and ∆FocGCS-C-GFP strains on PDA plates. Photos were taken after incubation at 28 °C for 5 days. c Colony diameters of the WT, ∆FocGCS, and ∆FocGCS-C-GFP strains grown on PDA plates. The mean values ± SD were calculated from three independent experiments, and asterisks indicate significant differences from the WT group (Student’s t-test, ***P < 0.001)