Fig. 3From: Loss of glucosylceramide synthase impairs the growth and virulence of Fusarium oxysporum f. sp. cubenseTargeted disruption of FocGCS affects conidiation, conidial germination, and conidial morphology of Fusarium oxysporum f. sp. cubense tropical race 4. a Conidial morphology of the WT, ∆FocGCS, and complemented strains. Blue fluorescence represents the number of fungal septa. Scale bars = 10 μm (upper) and 100 μm (bottom). b Conidial length examined by counting of at least 100 randomly selected conidia per three microscopy fields. The mean values ± SD were calculated from three independent experiments. c Conidia production of the indicated strains incubated for 5 days on PDA plates. d Conidial germination rate of the strains incubated on YEPD broth for 12 h. Germination of 100 conidia was randomly examined. Asterisks indicate significant differences from the WT group (Student’s t-test, ***P < 0.001)Back to article page