Fig. 4

Contents of glucosylceramides (GlcCers), hydroxyceramides (hCers), ceramides (Cers), and long chain bases (LCBs) in the WT and ∆FocGCS strains measured using HPLC–MS/MS. a Main glucosylceramide (GlcCer) levels in the WT and ∆FocGCS strains. b Main hydroxyceramide (hCer) levels in the indicated strains. c Total sphingolipid levels, including total LCBs, Cers, hCers, and GlcCers, in the indicated strains. d HPLC–MS/MS chromatograms showing main glucosylceramide retention in cultures of the indicated strains. The mean values ± SD were calculated from three independent experiments, and asterisks indicate significant differences from the WT group (Student’s t-test, **P < 0.01, ***P < 0.001)