Fig. 6

Cell wall integrity analysis of the WT, ∆FocGCS, and complemented strains. a Colony morphology of the indicated strains on PDA plates supplemented with 0.2% Calcofluor white (CFW) or 0.2% Congo red (CR). Photos were taken after incubation at 28 °C for 5 days. b The growth inhibition rate based on a. The inhibition rate was calculated by comparing the colony diameter under stress conditions with that under normal conditions. c Cellular chitin levels of the indicated strains measured by colorimetric determination of β-(1–4)-linked N-acetylglucosamine (GlcNAc). DW, dry weight. d Transmission electron micrographs of the WT and ∆FocGCS strains. CW, cell wall; M, mitochondrion; N, cell nucleus; V, vacuole. Scale bars = 0.2 μm. e Cell wall thickness of the indicated strains measured by 100 spores. The mean values ± SD were calculated from three independent experiments, and asterisks indicate significant differences from the WT group (Student’s t-test, *P < 0.05, **P < 0.01, and ***P < 0.001)