Fig. 8
Transcriptomic analysis to identify differentially expressed genes (DEGs) between the UvbZIP6-knockout mutant and the wild-type strain JS60-2. a Volcano plot of DEGs. b, c Gene ontology enrichment analysis of the DEGs, showing up-regulated DEGs in b and down-regulated DEGs in c. d Heatmap of FPKM-normalized transcript levels of the 565 DEGs in each pair-wise comparison. e, f Top 20 up-regulated (e) and down-regulated DEGs (f) in the KEGG enrichment analysis. g qPCR analysis to validate the DEGs. The relative transcript level of each gene from ∆UvbZIP6 was normalized by comparison with the gene from JS60-2 (relative transcript level = 1.0). Asterisks indicate the significant differences between the mutant and JS60-2 (P < 0.05). Error bars represent standard deviations