Fig. 2

GmPAO promotes PAMP (flg22 or chitin)-induced H₂O₂ accumulation. a, b GmPAO promoted PAMP (flg22 or chitin)-induced H₂O₂ accumulation in transgenic soybean lines. The leaves (2-week-old) were subjected to flg22- (a) or chitin-induced (b) ROS examination, and the relative luminescence unit (RLU) value was recorded. Error bar represents mean ± SD, n ≥ 8, n represents sample number. c, d GmPAO promoted PAMP (flg22 or chitin)-induced H₂O₂ accumulation in N. benthamiana. The indicated constructs were transiently expressed by Agrobacterium-mediated transient expression for 2 days and subjected to flg22- (c) or chitin-induced (d) ROS examination. GFP was used as a control. The protein expression is shown in the right panel. Error bar represents mean ± SD, n = 8, n represents sample number. All the experiments were performed three times (biological replicates) with similar results. The data were analyzed by Shapiro–Wilk test to determine the Normality and Lognormality Tests across groups, and then analyzed by unpaired t test for groups that had passed the normality test (**P < 0.01). The exact n, SD values, and P values are shown in the data