Fig. 1

Purification of RSV. a SDS-PAGE analysis of the purified RSV RNPs. Lane 1, crude RSV preparations obtained previously (Lin et al. 2020) were used as a control; Lane 2, RSV RNPs purified in this study. 10 µL of RSV preparations were loaded into each well. The gel was stained with Coomassie brilliant blue after electrophoresis. The arrows indicate the band with a size corresponding to RSV NP. M, molecular maker. b Western blotting analysis of the NP of RSV. After separation by SDS-PAGE, the proteins of the RSV preparations were transferred onto a nitrocellulose membrane and the blot was probed with an antiserum against RSV NP followed by a goat anti-rabbit IgG conjugated with HRP. c Observation of RSV via transmission electron microscopy. Red arrows indicate representative RSV RNPs. Scale bar, 100 nm