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Fig. 3 | Phytopathology Research

Fig. 3

From: CRISPR/Cas9-targeted mutagenesis of a representative member of a novel PR10/Bet v1-like protein subfamily significantly reduces rice plant height and defense against Meloidogyne graminicola

Fig. 3

Expression of OsBet v1 in rice tissues and in response to Meloidogyne graminicola invasion and subcellular localization of OsBet v1. a Expression of OsBet v1 in different rice organs. The level of OsBet v1 transcript at the shoots was set as 1 and used to calculate the relative expression level of this gene in other tissues. b Expression of OsBet v1 in rice roots infected with M. graminicola at 2 and 7 days post-inoculation (dpi). c Subcellular localization of OsBet v1. OsBet v1:eGFP was transformed into rice root protoplasts, with transformation of eGFP alone as the control. ARF19:RFP was used as a nuclear-localized marker. Scale bars: 20 μm. d Western blotting detection of the target proteins extracted from transformed cells. Protein bands with expected size of approximately 44 kDa for OsBet v1:eGFP and 27 kDa for eGFP were observed. eGFP, enhanced green fluorescent protein; RFP, red fluorescent protein. Data are presented as the mean ± SD. *P < 0.05, Student’s t-test

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Phytopathology Research

ISSN: 2524-4167