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Fig. 5 | Phytopathology Research

Fig. 5

From: CRISPR/Cas9-targeted mutagenesis of a representative member of a novel PR10/Bet v1-like protein subfamily significantly reduces rice plant height and defense against Meloidogyne graminicola

Fig. 5

Detection of ‘transgene-free’ homozygous OsBet v1 knockout mutants. Cas9 (722 bp), part of the Cas9-coding sequence; sgRNA (720 bp), a region spanning from the sgRNA to the downstream vector; Os18S rRNA (450 bp) was used as a normalization control; M, DL2000 DNA ladder; N, negative control with sterilized ddH2O as template; V, the plasmid vector; WT, wild-type plant. Lanes 1–11, 11 mutant individuals of OsBet v1#1. Lanes 12–22, 11 mutant individuals of OsBet v1#4

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Phytopathology Research

ISSN: 2524-4167