Fig. 6

The septin gene deletion mutants display distinct sensitivities to oxidative and nitrosative stress. a Reverse transcription-quantitative PCR analyses of the expression levels of the four septin genes VdSep3, VdSep4, VdSep5, and VdSep6 in XS11 in response to H₂O₂ stress. Gene expression values were normalized against an endogenous control (β-tubulin gene). Error bars represent standard deviation based on three independent replicates (**, P < 0.01). b Colony growth assay under stress by 15% H₂O₂ (upper panel) or 30% H₂O₂ (lower panel). Conidial suspension of each indicated strain was mixed with melted potato dextrose agar (PDA) medium to a final concentration of 10⁷ conidia/mL and poured into a petri dish to prepare plates. A sterile filter paper disk (5 mm in diameter) was transferred to the center of the plate and 5 μL H₂O₂ (15% or 30%) was then added to each paper disc. The diameters of the inhibition zone were measured after incubation at 25 °C for 3 days. Bar = 2 cm. c Statistic analysis of growth inhibition in b. Error bars represent standard deviation based on three independent replicates (**, P < 0.01; *, P < 0.05). d Colony growth assay in the presence of sodium nitroprusside dehydrate (SNP, 10 mM). All the strains were grown on complete medium (CM) or CM containing 10 mM SNP for 10 days. Bar = 2 cm. e Statistical analysis of growth inhibition in d. Error bars represent standard deviation based on three independent replicates (**, P < 0.01; *, P < 0.05)