Fig. 1
From: 3′ UTR is critical for viral RNA accumulation of jasmine virus H
Molecular characterization of JaVH infectious clone. a Schematic representation of the infectious viral clone generated in the pXT binary vector. The PCR-amplified full-length of JaVH-FJ was inserted into Stu I and BamH I digested binary vector. Open reading frames were identified in silico after sequencing of the full virus genome. Proteins are named according to homologous proteins in other pelarspoviruses. Predicted start and stop codons are indicated. Positions of duplicated 35S promoter, transcriptional start site, hepatitis delta virus antigenomic ribozyme (HDV RZ), and NOS terminator are indicated. b Symptoms of Nicotiana benthamiana Agro-infiltrated with infectious clone pXT-JaVHFJ and field jasmine sample from FuJian. c Detection of genomic RNA, subgenomic RNA and virus derived siRNA of JaVH by Northern blotting and coat protein (CP) by Western blotting. Healthy N. benthamiana leaves served as mock and Jasmine sambac leaves from Fujian Province containing JaVH served as positive control. A size marker and loading control are shown for reference. d, e Characterization of JaVH small RNAs in J. sambac (d) and N. benthamiana (e), the frequency of occurrence of each nucleotide at the 5′ end, the polarity and the size distribution of small RNAs are presented. f, g The distribution of small RNAs mapped to the JaVH genome is presented normalized to reads per million of total small RNAs in J. sambac (f) and N. benthamiana (g)