Fig. 5
Effect of MIP1 on the Tm-22-mediated disease resistance against ToMMV. a RT-qPCR analysis of the NbMIP1 mRNA accumulation levels in the silenced and the control Tm-22 transgenic Nicotiana benthamiana plants inoculated with TRV-derived vector at 10 days post-agro-infiltration (dpai). b The symptoms of the control and NbMIP1-silenced Tm-22 transgenic N. benthamiana plant inoculated with ToMMV at 7 dpai. c, d RT-PCR detection of the ToMMV RNA accumulation (c) and Western blot analysis of ToMMV CP accumulation (d) in the systemic leaves of NbMIP1-silenced and the control Tm-22 transgenic N. benthamiana plant. e Silencing efficiency of SlMIP1 gene in Jili tomato plants analyzed by RT-qPCR at 10 dpai. f The symptoms of SlMIP1-silenced Jili tomato and the control plants inoculated with ToMMV at 14 dpai. g RT-PCR detection of ToMMV RNA in the systemic leaves of SLMIP1-silenced and control Jili tomato plants inoculated with ToMMV at 14 dpai. h Western blot analysis of ToMMV coat protein (CP) in systemic leaves of the SlMIP1-silenced and the control plants at 14 dpai. The UBI gene served as an internal control for the RT-PCR and RT-qPCR analysis. The values indicate the mean + SD of three biological replicates. Letters indicate the significant differences in MIP1 accumulation between the MIP1-silenced and the control plants (t-test, P < 0.05). Yellow arrows show symptomatic systemic leaves and white arrows show asymptomatic systemic leaves