Fig. 6

SMV-RFP accumulation as observed via the presence of the red fluorescence on different soybean cultivars inoculated with pSC3-RFP. a The injured area was observed by YFP fluorescence and RFP fluorescence by simulating (buffer only) rubbing of inoculated control leaves. Scale bars, 1 mm. b The pSC3-RFP plasmids were directly rub-inoculated onto one spot of each leaf of the soybean cultivar NN1138-2 using a glass rod. Rescued SMV-RFP can accumulate at the inoculation site. The progression of the viral spread through the leaf vein was captured at 5, 6, 7, 8, 9, and 10 days post-inoculation (dpi) via a fluorescent stereoscope. c Soybean cultivars Zaoshu No.18 and Davis could restrict SMV-RFP spread within the leaf areas but supported SMV-RFP replication at the inoculation sites. d SMV-RFP could not replicate in the inoculated leaves from the soybean cultivars Qihuang No. 1, Dabaima, and Kefeng No. 1 as indicated by the lack of red fluorescence at the inoculation site. The images shown are representative ones from at least five repeated experiments