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Table 1 Primer sequences used for amplifying SMV-SC3 genome sequences

From: Construction and characterization of the infectious cDNA clone of the prevalent Chinese strain SC3 of soybean mosaic virus

Scheme

Forward primer (5' → 3')

Reverse primer (5' → 3')

35S

TCGAGCGGCCGCCTACTCCAAGAATATCAA

TTTATGAGTAGTTTTAATTTCCTCTCCAAATGAAATGAAC

A

GTTCATTTCATTTGGAGAGGAAATTAAAACTACTCATAAA

GGTACCCAACTGTTAAAGATCCAAA

B

TTTTGGATCTTTAACAGTTGGGTAC

CCGGTTATCTGTATCAAGAGTAGCC

C

GGCTACTCTTGATACAGATAACCGG

GGCCCATGCCCAGAAGAGTGTGCAT

D

ATGCACACTCTTCTGGGCATGGGCC

GCTATCGATTTTTTTTTTTTTTTTTTTTTTT

YFP

TCCCCCGGGGTGAGCAAGGGCGAGGAGCTGT

CGACGCGTCTTGTACAGCTCGTCCATGCC

  1. The primers were designed according to the full-length reference genome sequence of SMV-SC3. The adjacent PCR fragments overlap to each other with a 25 bp long homologous arm
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Phytopathology Research

ISSN: 2524-4167