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Fig. 1 | Phytopathology Research

Fig. 1

From: The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

Fig. 1

Deletion of MoB56 in Magnaporthe oryzae. a Comparison of conserved domain diagrams among the regulatory B56 ortholog protein sequences in selected species. NCBI accession numbers are as follows: Arabidopsis thaliana epsilon isoform of B56, AtB’ε, NP_001030770.1; Caenorhabditis elegans CeB56, NP_001122845.1; delta isoform of B56 from Homo sapiens, HsB’δ, KAI2542369.1; Schizosaccharomyces pombe SpPar1, NP_588206.1; S. pombe SpPar2, NP_593298.1; Saccharomyces cerevisiae ScRts1, NP_014657.1; Ustilago maydis UmB56, XP_011389548.1; M. oryzae MoB56, XP_003719828.1; Fusarium graminearum FgB56, XP_011324500.1; Sclerotinia sclerotiorum SsB56, XP_001591242.1; Neurospora crassa NcB56, XP_961132.2; Aspergillus nidulans ParA, XP_868849.1. b Gene deletion strategy of MoB56 in M. oryzae. Restriction enzymes used for constructing the pKOV-MoB56 replacement vector are labeled by BamHI (B), SpeI (Sp), HindIII (H) and XhoI (X). c PCR amplification of MoB56 gene in the wild-type (WT) and knockout strains. d Southern blot assay of Δmob56, genomic DNA of the wild-type and mutant strains were digested with SacI (S) as shown in b

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Phytopathology Research

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