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Fig. 5 | Phytopathology Research

Fig. 5

From: Candidatus Phytoplasma ziziphi encodes non-classically secreted proteins that suppress hypersensitive cell death response in Nicotiana benthamiana

Fig. 5

The HR-suppressing Pzi-ncSecPs enhanced gene expression of cell death inhibitors in Nicotiana benthamiana. The N. benthamiana leaves were individually inoculated with the agrobacterial cells bearing the Pzi-ncSecP or the GFP genes, and further challenged with Bax or INF1 at 24 h post-inoculation (hpi). The leaves were harvested at 24 h post-Bax or INF1 infiltration, followed by the total RNA extraction. The relative transcriptional levels of three pathogenesis-related genes (NbPR-1, NbPR-2, and NbPR-5) (a) and two Bax inhibitor-1 genes (NbBI-1 and NbBI-2) (b) were analyzed by using RT-qPCR. Three biological replicates were performed, each with three technical replicates. The N. benthamiana actin gene was employed as the internal reference. Bars indicate the standard errors of the mean, and asterisks show statistically significant differences based on Student’s t-tests (*, P < 0.05, **, P < 0.01)

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Phytopathology Research

ISSN: 2524-4167