Fig. 1

Fg62 induced cell death in Nicotiana benthamiana. a Cell death detection on N. benthamiana leaves after transient expression of eight candidate effectors, green fluorescent protein (GFP), and PsXEG1. Candidate proteins were derived from the secretome of Fusarium graminearum PH-1 and possessed signal peptides and unknown functional domains. Representative images were taken at 7 days post-inoculation (dpi). b Quantification of cell death by electrolyte leakage measurement. Electrolyte leakage from infiltrated leaf discs was measured as a percentage of leakage from boiled discs. Means and standard errors (SEs) of three independent experiments are shown. Different letters above the bars indicate significant differences according to one-way analysis of variance (ANOVA), followed by Tukey’s test (P < 0.05)