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Fig. 3 | Phytopathology Research

Fig. 3

From: A potential biocontrol agent Streptomyces tauricus XF for managing wheat stripe rust

Fig. 3

Effects of XF on Pst growth in leaves. a Histological observation of fungal growth in wheat. SV, substomatal vesicle; IH, infectious hyphae; HMC, haustorial mother cell. Bars = 20 μm. b Infection area, the average area of the expanding hypha plus the host cells, was calculated by CellSens Entry software. c Hyphal length is the average distance from the junction of the substomatal vesicle and the hypha to the tip of the hypha. d Haustoria formation rate of Pst with various treatments. e RT-qPCR analysis of the relative expression levels of PstEF in leaves at 72 h after inoculation with Tr, AC, and FL. TaEF and PstEF were used to normalize the RNA level of wheat leaves and Pst, respectively. SDW, sterile distilled water; Tr, 20% triadimefon emulsions at a concentration of 0.5 g/L; AC, XF actinomycete cell suspension (107 CFU/mL); FL, XF fermentation liquid without actinomycete cells. Values in charts are mean ± SD. An asterisk indicates significant differences based on the unpaired two-tailed Student’s t-test with the P values marked (*P < 0.05; **P < 0.01) between the SDW treatment and other treatments

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