Fig. 5
From: Leafhopper transmits soybean stay-green associated virus to leguminous plants
Bean bug (Riptortus pedestris) cannot transmit SoSGV. a The experimental setup of SoSGV transmission assay. The insects were reared on soybean seedlings in a nylon cage. No other food sources were supplied. b The time flow chart of two SoSGV transmission experiments. c PCR detecting SoSGV in soybean seedlings infested or non-infested by R. pedestris. SoSGV was amplified using primer pair #3133 and #3144. The gene coding for soybean β-tubulin (GmTUBB) was amplified via PCR as a loading control. Water and a plasmid containing SoSGV genome DNA were used as negative (N) and positive (P) controls, respectively. Different lanes represent different plants tested using PCR. d quantitative PCR analysis of the same samples in panel c. e Detection of SoSGV in R. pedestris via PCR. The R. pedestris adults were either reared on soybean seedlings or on dry soybean seeds at the indicated time. The gene coding for R. pedestris actin (RpActin) was used as a loading control. f PCR detecting SoSGV in soybean seedlings infested or non-infested by R. pedestris in the 2nd experiment. See further detail in panel c