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Fig. 7 | Phytopathology Research

Fig. 7

From: Chitin synthases containing myosin motor-like domain are required for cell wall integrity and virulence of vascular wilt pathogen Verticillium dahliae

Fig. 7

Effects of VdChs5 and VdChs7 HIGS on cotton resistance to Verticillium dahliae V592 infection. a Disease symptoms of cotton seedlings (TM-1) infiltrated with different HIGS constructs. The seedlings were photographed at 30 days post inoculation (dpi) with V592. b The transverse section of stems of different HIGS-treated cotton plants at 30 dpi. Scale bars, 0.5 mm. c The longitudinal section of stems of different HIGS-treated cotton plants at 30 dpi. Scale bars, 0.5 mm. d Fungal recovery from infected stems of different HIGS-treated cotton plants at 30 dpi. e The disease grade of different HIGS-treated cotton plants at 30 dpi. f The disease index of different HIGS-treated cotton plants at 30 dpi. g The relative fungal biomass of different HIGS-treated cotton plants at 30 dpi. h The relative expression levels of VdChs7 in V592-infected cotton plants injected with diverse TRV constructs. i The relative expression levels of VdChs5 in V592-infected cotton plants inoculated with distinct TRV constructs. For e–i, data represent means and standard deviations from three independent biological replicates. *** indicates significant differences at P < 0.001 as calculated with Student’s t-test

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