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Fig. 4 | Phytopathology Research

Fig. 4

From: Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases

Fig. 4

Optimal conditions of the CPA reaction system. a Schematic representation of primer positions in P. infestans contig 1.18131. A set of five candidate primers were represented respectively. 2a1s: cross primer. 4s, 5a: displacement primers. b and c Determination of CPA products derived from primer sets through 2% agarose gel electrophoresis with 10 μg pure DNA of P. infestans (1 μL of each) for indicated reaction time or at annotated temperature, respectively. Quantification and analysis of DNA product intensity were performed with ImageJ software. A.U., Arbitrary Unit

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Phytopathology Research

ISSN: 2524-4167