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Fig. 5 | Phytopathology Research

Fig. 5

From: Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases

Fig. 5

Specificity test and applicability analysis of developed CPA for P. infestans detection. a and b Colorimetric assessment and gel electrophoresis analysis of the CPA amplificons. Number 1 to 16 indicated P. infestans T30-4, P. infestans 88069, P. infestans MZ, P. capsica, P. nicotianae, P. melonis, P. drechsleri, P. cryptogea, P. parasitica, P. cactorum, Py. ultimum, B. cinerea, F. culmorum, F. solani, R. solani, and E. carotovora, respectively. N, negative control. c Applicability evaluation of CPA through simulating the complex sample environment in the field. P.i, P. infestans. B.c, B. cinerea. P.c, P. capsica. N, negative control, 0% P. infestans. d, f Representative potato tubers and leaves (after trypan blue staining) were infected with P. infestans. Region I to VI represented artificially devised areas varying by stages of infection. e, g Detection of P. infestans using CPA with DNA extracted from Region I to VI. N, negative control. P, positive control, pure DNA of P. infestans

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Phytopathology Research

ISSN: 2524-4167