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Fig. 7 | Phytopathology Research

Fig. 7

From: Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases

Fig. 7

Detection of diseased field samples using SMA-Device extraction and visual CPA assay. a Typical P. infestans-infected potato leaves with varying degrees of severity. b A fragment of the P. infestans genome was amplified by PCR using DNA extracted from a following the SMA-Device extraction. c Diagnosis of P. infestans with visual CPA assay using the corresponding DNAs. d Photographs of samples with representative symptoms of potato late blight, tomato gray mold, and pepper Phytophthora blight, respectively. e Challenging plant leaves collected from the field were subjected to SMA-Device extraction and visual CPA assay, and the detection rate was displayed

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