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Fig. 2 | Phytopathology Research

Fig. 2

From: 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae

Fig. 2

XooFabH1 encodes a 3-ketoacyl-ACP synthase III to initiate FASII. a SDS-PAGE analysis of purified XooFabHs. b Condensation of malonyl-ACP with acetyl-CoA by purified EcFabH (lane 3), XooFabH1 (lane 4), XooFabH2 (lane 5), or XooFabH3 (lane 6). The migration positions of octanoyl-ACP (C8:0-ACP, lane 7) on gel are shown. c Condensation of malonyl-ACP with isobutyryl-CoA (lane 1) or isovaleryl-CoA (lane 3) by purified FabH1. The migration positions of octanoyl-ACP (C8:0-ACP, lane 4) on gel are shown. d Condensation of malonyl-ACP with acyl-CoAs (C4:0-CoA to C12:0-CoA) by purified XooFabH1 (lane 3 to lane 7). The migration positions of hexanoyl-ACP (C6:0-ACP, lane 8) and octanoyl-ACP (C8:0-ACP, lane 9) on gel are shown. The initiation fatty acid synthesis was reconstructed using a combination of E. coli FabZ, FabG, FabI, and Xoo FabH1 with NADH, and NADPH as cofactors, and malonyl-ACP plus acetyl-CoA or isoacetyl-CoA as substrates

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Phytopathology Research

ISSN: 2524-4167