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Table 1 Fatty acid composition of ΔRsfabH complementary strains and Xoo mutant strains

From: 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae

Strain

n-C14:0

iso-C15:0

n-C16:0

n-C16:1

iso-C17:0

anteiso-C17:0

n-C18:0

n-C18:1

GMI1000%

1.5 ± 0.3

–

29.5 ± 2.7

11.6 ± 1.4

–

–

22.4 ± 1.6

32.1 ± 3.3

ΔRsfabH/XoofabH1%***

1.0 ± 0.3

37.8 ± 2.3

9.2 ± 1.1

14.8 ± 2.1

6.4 ± 0.2

4.6 ± 0.6

18.7 ± 1.2

7.5 ± 1.5

Xoo%

1.26 ± 0.15

7.72 ± 0.05

41.64 ± 2.2

30.9 ± 2.72

8.72 ± 0.84

–

8.3 ± 1.11

1.46 ± 0.09

ΔfabH1%***

1.79 ± 0.3

0.17 ± 0.04

48.79 ± 0.92

37.37 ± 2.53

–

–

9.8 ± 1.94

2.08 ± 0.72

ΔfabH2%

1.14 ± 0.2

6.47 ± 0.1

39.39 ± 1.9

32.13 ± 0.3

9.22 ± 0.4

–

9.65 ± 1.3

2.01 ± 0.32

ΔfabH3%

1.26 ± 0.6

8.11 ± 1.7

41.7 ± 1.8

29.35 ± 1.2

8.61 ± 0.6

–

9.33 ± 1.6

1.63 ± 0.2

ΔfabH1/fabH1%*

1.61 ± 0.38

6.92 ± 2.25

38.97 ± 1.56

28.51 ± 0.58

13.66 ± 1.01

–

7.91 ± 2.53

2.41 ± 0.91

  1. Total lipids were extracted and transesterified to fatty acid methyl esters, and products were identified by GC–MS. Values are percentages of total fatty acids and are the mean ± standard deviations of three independent experiments. Pair-wise comparisons were made between the ΔRsfabH complementary strains and wild-type strain GMI1000, and the wild-type strain PXO99A and mutant strains or complemented strains, by Student’s t-test (***P < 0.001; **P < 0.01; *P < 0.05). n-C14:0, tetradecanoic acid; iso-C15:0, 13-methyl-tetradecanoic acid; n-C16:1, cis-9-hexadecenoic acid; n-C16:0, hexadecanoic acid; iso-C17:0, 15-methyl-hexadecanoic acid; anteiso-C17:0, 14-methyl-hexadecanoic acid; n-C18:0, octadecanoic acid; n-C18:1, cis-11-octadecenoic acid