Table 1 Primers used for the loop-mediated isothermal amplification assay
Primer name | Primer sequence (5'–3') | Length (nt) |
|---|---|---|
F3 (forward outer) | TCAGACTGATGGGTCATCT | 19 |
B3(backward outer) | AGATGAAACGTTGATCAAGATAA | 23 |
FIP (forward inner; F1c + F2) | TACAGAGGCAGTCCTGCTTG–TCAATAAATTAGCTTGAACTGAGTG | 45 |
BIP (backward inner; B1c + B2) | ACACAATCAATAACAGAGTGAGAGG–GCACTATTAAGAGAAGAAGACC | 47 |
LB (loop backward) | GATGACGTAAGGTACTGACACCT | 23 |