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Fig. 3 | Phytopathology Research

Fig. 3

From: A G-type lectin receptor-like kinase in Nicotiana benthamiana enhances resistance to the fungal pathogen Sclerotinia sclerotiorum by complexing with CERK1/LYK4

Fig. 3

NbERK1 associates with NbCERK1 and NbLYK4. a NbERK1 associates with NbCERK1 in Nicotiana benthamiana. GFP-tagged NbCERK1 and HA-tagged NbERK1 were co-expressed in N. benthamiana for 36–48 h. Samples were treated with 200 μg/mL chitin for 15 min before harvesting. Protein associations were detected by Co-IP assays. b NbERK1 associates with NbLYK4 in N. benthamiana. GFP-tagged NbLYK4 and HA-tagged NbERK1 were co-expressed in N. benthamiana for 36–48 h. Samples were treated with 200 μg/mL chitin for 15 min before harvesting. Protein associations were detected by Co-IP assays. c NbERK1 associates with NbLYK4 by a split-luciferase assay. NbERK1-Nluc was co-expressed with the indicated Cluc constructs in N. benthamiana. The intensity of protein interaction was shown by the relative luminescence unit (RLU), and protein expression was shown by immunoblotting (mean ± SD, n ≥ 6). d NbERK1 associates with NbLYK4 in the BiFC assay. NbERK1 and NbLYK4 were fused to the N terminus or C terminus of the YFP fragment, respectively. YFP fluorescence was detected by confocal microscopy. Bars, 25 μm. e NbERK1 associates with NbCERK1 in the yeast two-hybrid assay. Yeast cells with corresponding vectors were grown on SD-Leu/-Trp or SD-Ade/-His/-Leu/-Trp medium. AD and BD represent empty plasmids. ECD, extracellular domain; CD, cytosolic domain

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Phytopathology Research

ISSN: 2524-4167