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Fig. 3 | Phytopathology Research

Fig. 3

From: Crystal structure of rice APIP6 reveals a new dimerization mode of RING-type E3 ligases that facilities the construction of its working model

Fig. 3

Evaluation of the critical residues of APIP6-RING for homodimer formation with LCI assay. Only the four mutants, S50A, Q55A, S67A, and D53A/N70A that were proved to be critical for dimer formation with Y2H were further evaluated with LCI. Coding sequences of these mutants were cloned into vectors in-frame with NLuc and CLuc. E2 was cloned in-frame with NLuc and was used to evaluate whether these mutants hamper the interaction between APIP6-RING and E2

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Phytopathology Research

ISSN: 2524-4167