Fig. 2

CcEG1 induce plant defense response in N. benthamiana with signal peptide. a Verification of secretion activity of CcEG1 signal peptide by yeast signal trap assay. Yeast strain YTK12 cannot grow on medium using sucrose as the only carbon source, but when plasmid Psuc2 that lacking the signal peptide and the initiation codon ATG could ligate to a functional signal peptide the and then introduced into YTK12, the yeast strain can grow normal. The known functional SP of Avr1b was used as a positive control, Mg87 was set as negative control. b The cell death-inducing ability of CcEG1 was determined in N. benthamiana. BAX and pGR106 (EV) were chosen as positive and negative controls, respectively. Photographs were taken 5 days post agroinfiltration. c Western blot of proteins from N. benthamiana leaves transiently expressing CcEG1, CcEG1^ΔSP, PR1^SP-CcEG1^ΔSP fused with 3*HA tags. d Schematic diagram of CcEG1 with or without signal peptide, and replacing by pathogenesis-related protein 1 (PR1). e CcEG1 can induce plant immune defense response when it contains functional signal peptide. BAX and pGR106 (EV) were chosen as positive and negative controls, respectively. The picture was photographed 24 h after the infiltration. Data represented have three independent biological repeats