Fig. 4

CcEG1 has no effect on the pathogenicity of C. chrysosperma. a CcEG1 mutant was constructed by the method of Split marker to replace target gene with hygromycin cassette based on the principle of homologous recombination. b, c CcEG1 single-copy mutants were verified by internal and external primers and Southern blot. d, f Inoculated poplar branches or leaves with plugs of wild-type, CcEG1 mutants, and complemented strains, agar was set as negative control. The lesion areas were measured at 5 dpi. e, g Quantification of colony diameter in poplar twigs or leaves that inoculated with WT, CcEG1 mutants, and complemented strains. This experiment was performed three times with similar results, each assay was performed on at least three independent biological repeats. The statistical analyses were conducted by SPSS v16.0, and Duncan s test at P ≤ 0.05 or P ≤ 0.01 was used for determining the differences between mutants and WT strain. h Multiple sequence alignment of GH12 domain from functional characterized GH12 members