Fig. 6

The GH12 domain was sufficient for CcEG1 to induce cell death in N. benthamiana. a Schematic diagram of CcEG1 mutant. b Cell death-inducing activity of the CcEG1 mutant were observed 5 days after agroinfiltration in N. benthamiana, BAX, and pGR106 were used as positive and negative controls, respectively. c The detection of ROS and callose deposition in N. benthamiana leaves at 24 h post inoculation. d Western blot of proteins from N. benthamiana leaves transiently expressing CcEG1, CcEG1-Δcbm1 and SP-CBM1 with 3*HA tags