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Fig. 7 | Phytopathology Research

Fig. 7

From: A glycoside hydrolase 12 protein from Cytospora chrysosperma triggers plant immunity but is not essential to virulence

Fig. 7

CcEG1 requires the receptor-like kinases BAK1 and SOBIR1 to induce plant immune responses in N. benthamiana. a, d After three weeks silencing BAK1 and SOBIR1 by virus-induced gene silencing (VIGS), CcEG1 was inoculated in N. benthamiana leaves, and the results showed that it could not induce cell death. b, e The silencing efficiencies of BAK1 and SOBIR1 were determined by quantitative RT-qPCR. Three biological and statistical replicates were performed. c, f Western blot of CcEG1 protein fused with 3*HA tag that transiently expressed in BAK1 or SOBIR1 silenced N. benthamiana leaves. The total protein is shown by Coomassie brilliant blue (CBB). **P < 0.01, ***P < 0.001

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