Fig. 3

Validation of the differentially expressed (DE) circRNAs in rice plants after Xoo-infection. a Number of DE circRNAs after Xoo-infection were identified by find_circ and CIRI2 software. b Number of the up- and down-expressed circRNAs after Xoo-infection. c Number of the potential network prediction of circRNA-miRNA-mRNA. The DE circRNAs were used for predicting the target mRNAs and miRNAs. d–f Validation of circular RNA formation of 3 circRNAs by (RT)-PCR assays. d PCR diagram using genomic DNA (left) and circular cDNA (right) of circRNAs as template. e The electrophoretogram of circular RNA formation of 3 circRNAs by (RT)-PCR assays. The circular cDNA of circRNAs (ciR52, ciR298, and ciR133) could be amplified by divergent primers (first column), but not their genomic DNA (gDNA) (third column). Circular cDNA from circRNAs and gDNA of the parental genes could be amplified by the convergent primers (second and fourth columns). f Expression change of the DE circRNAs in ZH11 after Xoo infection using divergent primers in RT-qPCR assays. OseEF1-a was used as a reference gene. Means ± SD represent the value with standard deviation (n = 3 biological repeats)