Fig. 4

ciR133 is a non-coding circular RNA derived from OsARAB. a Schematic diagram of ciR133 RNAs formed by back-splicing from the 4th, 5th and 6th exons of its parental gene OsARAB. b Validation of circular RNA formation of ciR133 by RT-PCR assays. Total RNAs of rice seedlings were treated with RNase R, and then used for the RT-PCR assays. c RT-qPCR analysis for ciR133 expression level in the Xoo infected rice leaves. RT for total RNAs were performed with random or oligo(dT) primers. Os-eEF1-1a was used as a reference gene. Means ± SD represents the value with standard deviation (n = 3 biological repeats, **P ≤ 0.01)