Skip to main content
Fig. 5 | Phytopathology Research

Fig. 5

From: Xoo-responsive transcriptome reveals the role of the circular RNA133 in disease resistance by regulating expression of OsARAB in rice

Fig. 5

ciR133 represses expression of OsARAB and responds to Xoo infection. a Expression change of ciR133 and its parental gene OsARAB in four weeks old ZH11 plants after Xoo (AXO1947)-infection. b Dual-luciferase assay of interaction between ciR133 and OsARAB in tobacco leaves. Schematic diagram of interaction construction was displayed in upper panel. Interaction of ciR133 and OsARAB mRNA was displayed in left panel, and the luciferase activity was shown in right panel. N. benthamiana were grown at 30°C. The plants were infected for two days at 25°C. c Expression level of circular ciR133 in the transgenic rice lines with overexpression of circular ciR133 (ciR133-ox), down-expression of circular ciR133 (Si-ciR133), and overexpression of linear ciR133 (Linear133-ox). To confirm whether the circular ciR133 RNAs were altered in these transgenic rice seedlings (ciR133-ox and Si-ciR133), their total RNAs were digested with RNase R for RT, and the divergent primers were used for qPCR assays. Os-eEF1-1a was used as a reference gene. d Expression change of OsARAB in ciR133-ox, Si-ciR133, and Linear133-ox seedlings. Os-eEF1-1a was used as a reference gene. Means ± SD represents the value with standard deviation (n = 3 biological repeats)

Back to article page

Phytopathology Research

ISSN: 2524-4167