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Fig. 3 | Phytopathology Research

Fig. 3

From: Gene editing with an oxathiapiprolin resistance selection marker reveals that PuLLP, a loricrin-like protein, is required for oospore development in Pythium ultimum

Fig. 3

CRISPR/Cas9-mediated in situ PuLLP gene complementation using PcMuORP1 as a selection marker. a Schematic representation of plasmid modification and homologous recombination for PuLLP gene complementation. Cas9 and sgRNA were divided into two plasmids, and the NPTII gene was replaced with PcMuORP1. The location of the primers used to validate complementation and Sanger sequencing traces of junction regions confirming complementation of the PuLLP ORF are shown. b Analysis of genomic DNA from the wild type (WT), ΔPuLLP-EV (in which PuLLP-m was not successfully complemented), and PuLLP-complemented mutants (ΔPuLLP-C) using the primers shown above and actin primers as a positive control

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Phytopathology Research

ISSN: 2524-4167