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Table 2 Stability of the selection markers PcMuORP1 and NPTII in regenerated transformants

From: Gene editing with an oxathiapiprolin resistance selection marker reveals that PuLLP, a loricrin-like protein, is required for oospore development in Pythium ultimum

Selection marker

Number of mutants losing resistance

in every three generations*

Number of mutants maintaining resistance

3

6

9

12

15

18

21

PcMuORP1

0

0

8

3

0

0

0

4

NPTII

0

0

3

0

4

2

0

6

  1. *In each case, 15 Py. ultimum transformants were inoculated on V8 plates without selection pressure. After three generations, the transformants were transferred back to the plates amended with oxathiapiprolin or G418 to determine whether the transformants lost resistance
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Phytopathology Research

ISSN: 2524-4167