Fig. 3

Production of perithecia in F. verticillioides. a The LNF15-11 isolate (MAT-2) was used as the female parent and crossed with the 18 MAT-1 isolates. The mature perithecia formation on carrot agar media after 4 weeks of fertilization. The sexual reproductive capacity of each isolate was variable. Among them, the JLF19-41, SXF20-2, and SHF19-37 isolates generate the most perithecia. b Statistical analysis of the number of perithecia in each case. c Statistical analysis of conidia production of different F. verticillioides isolates. The isolates were cultured on PDA for 6 days. One agar plug (1 cm in diameter) was collected from the edge of the colony and placed in 1 mL of sterile water. Conidia were released by vortexing and counted with a hemacytometer. d Correlation analysis between the sporulation and production of perithecia. Data represent the mean of three independent biological replicates. Bars indicate standard error. One-way ANOVA was used for statistical analysis. Columns with different letters represent a statistical significance of P < 0.05 according to the Waller-Duncan test