Fig. 3

Single-nucleotide resolution maps and semiquantitative RT-PCR analysis of vsiRNAs. a Schematic diagram of SCMV genome. b The single-nucleotide resolution maps of 21- and 22-nt vsiRNAs on SCMV genome in SM and SC libraries. The SCMV genome was arranged along the X-axis with length drawn to scale. vsiRNAs derived from the sense and antisense strand of SCMV genomic RNAs were shown above and below the horizontal line, respectively. The data used to generate the charts were based on the means calculated from three libraries for each treatment. c Semiquantitative RT-PCR analysis of vsiRNAs in Mock- and SCMV-inoculated Mo17 and Chang7-2 maize plants. Semiquantitative RT-PCR assays were conducted with three independent biological replicates using Mock- and SCMV-inoculated maize plants. “(+)” indicates vsiRNAs derived from the sense strand of SCMV genomes, and “(−)” indicates the antisense strand. U6 was used as the internal control. MM, Mo17 inoculated with phosphate buffer; SM, Mo17 inoculated with SCMV; MC, Chang7-2 inoculated with phosphate buffer; SC, Chang7-2 inoculated with SCMV