Fig. 1

The putative effector Pb257 in P. brassicae induced cell death and ROS burst in Nicotiana benthamiana leaves. a, b Pb257-infiltrated leaves exhibited cell death at 3 days post-inoculation (dpi), and ROS burst at 1 dpi. GFP was infiltrated as a negative control (CK-). BAX, a mammalian proapoptotic BCL-2 associated X (Bax) protein, was infiltrated as a positive control (CK+), respectively. The experiment was replicated ten times. c Expression of Pb257 proteins in infiltrated leaves detected by Western blotting. The anti-GFP antibody was used. The arrow points to the Pb257 protein. d The protein electrophoresis gel was stained with Coomassie brilliant blue