Fig. 5

Analysis of dependent genes and pathway of Pb257 induced cell death. a Representative photographs of Pb257-induced cell death in A. thaliana eds5, npr1, bak1-4, NahG mutant leaves, and wild-type leaves at 4 days post-infiltration. Numbers e.g., 14/15, indicate that 14 of 15 infiltrated leaves exhibiting cell-death. b Representative photographs of Pb257-induced cell death in silenced N. benthamiana leaves at 4 days post-infiltration. Pb257 was transiently expressed in N. benthamiana leaves silenced for pTV00 (control), BAK1, or SOBIR1. GFP was control proteins. The experiment was replicated ten times. c The transcript expression levels of SOBIR1 and BAK1 in the corresponding infiltration area of leaves were took to analyze by qRT-PCR. Statistical analysis was performed by one-way ANOVA, followed by Tukey’s multiple comparison test. Error bars are the standard deviation (SD) of three independent replicates (**p < 0.01)